Journal: Cell reports

Article Title: Mass Cytometry Reveals Global Immune Remodeling with Multi-lineage Hypersensitivity to Type I Interferon in Down Syndrome

doi: 10.1016/j.celrep.2019.10.038

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-human CD45RO (clone UCHL1) , Fluidigm , Cat#3164007B; RRID:AB_2811092.

Techniques: Recombinant, Mass Cytometry, Software

Journal: Immunity

Article Title: Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions

doi: 10.1016/j.immuni.2021.06.002

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal Anti-Human CD45RO (UCHL1)-173Yb antibody , Fluidigm , Cat# 3173016D; RRID: AB_2811052.

Techniques: Recombinant, Purification, Functional Assay, Control, Blocking Assay, Modification, Microscopy, Staining, Antibody Labeling, Mass Cytometry, Software, Cytometry, Plasmid Preparation

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: The Art and Science of Selecting a CD123-Specific Chimeric Antigen Receptor for Clinical Testing

doi: 10.1016/j.omtm.2020.06.024

Figure Lengend Snippet: CD123-CAR CD20 T Cells Have Similar Kinetics and Immunophenotypes (A) Fold expansion of the CD123-CAR CD20 and non-transduced (NT) T cells (n = 5; p ≥ 0.05). (B) Viability of the indicated populations was determined by acridine orange/propidium iodide (AO/PI) exclusion (n = 5; p ≥ 0.05). (C and D) Immunophenotype and exhaustion phenotype was determined by flow cytometry on day 8. (C) T cell immunophenotypes (T cell subsets: naive, CCR7 + CD45RO – ; central memory (CM), CCR7 + CD45RO + ; terminally differentiated (TD), CCR7 – CD45RO – ; and effector memory (EM), CCR7 – CD45RO + ; n = 5. (D) CD4, CD8, Tim3, and PD1 expression (n = 5; p > 0.05 among CD123-CAR T cell groups).

Article Snippet: The following antibodies were used: CD4 (Clone OKT4, BV785, BioLegend; 317442), CD8 (Clone SK1, APC-Cy7, BD PharMingen; 557834), CCR7 (Clone REA546, PE, Miltenyi Biotech; 130-108-285), CD45RO (Clone UCHL1, APC, Tonbo; 20-0457-T100), Tim3 (Clone F38-2E2, PE-Cy7, Biolegend; 345014), PD1 (Clone EH12.2H7, BV421, Biolegend; 329920), CD20 (Clone 2H7, FITC, Tonbo; 35-0209-T100), and goat anti-human Fc-IgG (pooled goat antisera, PE, Southern Biotech; 2048-09).

Techniques: Flow Cytometry, Expressing

Journal: Cell

Article Title: Enhanced safety and efficacy of protease-regulated CAR-T cell receptors

doi: 10.1016/j.cell.2022.03.041

Figure Lengend Snippet: bd

Article Snippet: Anti-CD45RO (clone UCHL1) , Fluidigm , 3149001B.

Techniques: Purification, Virus, Recombinant, Membrane, Transfection, Enzyme-linked Immunosorbent Assay, Antibody Labeling, Flow Cytometry, Luciferase, Software, Imaging